Download PDF by Richard Nuccitelli, Leslie Wilson, Paul T. Matsudaira: A Practical Guide to the Study of Calcium in Living Cells

By Richard Nuccitelli, Leslie Wilson, Paul T. Matsudaira

ISBN-10: 0125228104

ISBN-13: 9780125228107

ISBN-10: 0125641419

ISBN-13: 9780125641418

A Practical advisor to the learn of Calcium in residing Cells describes renowned recommendations besides necessary do's and don't's and laptop courses. the quantity allows investigators to judge confocal photos, use the newest dyes, and layout Calcium buffers acceptable to their learn wishes. This booklet is designed for laboratory use by way of graduate scholars, technicians, and researchers in lots of disciplines, starting from molecular to mobile degrees of research. Key positive aspects * Describes thoughts for detection of [Ca2+]I: Ca2+ - delicate microelectrodes * Fluorescent dyes * Luminescent proteins * comprises recommendations for perturbing intracellular Ca2+ * Covers distinct technique plus difficulties and pitfalls of every strategy * encompasses a sensible consultant to getting ready Ca2+ buffers with an easy-to-use machine application * colour plates illustrate suggestions reminiscent of * Confocal ratio-imaging * Use of aequorin

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Extra resources for A Practical Guide to the Study of Calcium in Living Cells

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Harafuji, H.. and Ogawa, Y. (1980). N’,N‘-tetraceticacid with calcium around neutral pH. J. Biochem. 87, 1305-1312. Harrison, S. , and Bers. D. M. (1987). The effect of temperature and ionic strength on the apparent Ca-affinity of EGTA and the analogous Ca-chelators BAPTA and dibromo-BAPTA. Biochim. Biophys. Acta 925, 133-143. Harrison, S. , and Bers, D. M. (1989). Correction of absolute stability constants of EGTA for temperature and ionic strength. Am. J . Physiol. 256, C125O-Cl256. Hellam, D.

Bound and determined: A computer program for making buffers of defined ion concentrations. Anal. Biochem. 201, 119-126. Fabiato. A. (1988). Computer programs for calculating total from specified free or free from specified total ionic concentrations in aqueous solutions containing multiple metals and ligands. Meth. Enzymol. 157, 378-417. Fabiato. A. (199 I). Ca’+ buffering: Computer programs and simulations. In “Cellular Calcium: A Practical Approach” ( J . G. McCormack and P. H. Cobbold. ), pp.

Some of the file formats are given for individuals who want to write their own accessories. The source code is omitted so users will be forced to contact the programmer (C. W. Patton) to make changes (including bug fixes) that can then be passed on to other registered users. D. How to Obtain MAXC and Spreadsheets To obtain MAXC and the spreadsheets described here, send $10 to cover postage, duplicating, and packaging costs to: Chris Patton, Stanford University, Hopkins Marine Station, Pacific Grove, California 93950-3094 Please specify disk size and capacity (-400 kB is needed for all the uncompressed files).

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A Practical Guide to the Study of Calcium in Living Cells by Richard Nuccitelli, Leslie Wilson, Paul T. Matsudaira

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