By Burkhard Ludewig, Matthias W. Hoffmann
An authoritative choice of optimum options for generating and characterizing the immunologically energetic cells and effector molecules now gaining extensive use within the medical remedy of sufferers. benefiting from the most recent applied sciences, the authors current without difficulty reproducible experimental protocols for the research of dendritic cells, T cells, monoclonal antibodies, and bone marrow transplantation. The emphasis is on preclinicical and medical functions and at the development of chosen methods in scientific trials. extra chapters hide the molecular definition of aim antigens, mathematical modeling ways to immunotherapy, and the usage of regulatory T cells. The protocols give the chance to check the adoptive move of adapted antigen-specific immune cells and to enhance the medical software of adoptive immunotherapy.
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Additional resources for Adoptive immunotherapy : methods and protocols
2002) Blood dendritic cells interact with splenic marginal zone B cells to initiate T-independent immune responses. Immunity 17, 341–352. 47. , Palucka, A. , Blanck, J. , and Banchereau, J. (2003) Plasmacytoid dendritic cells induce plasma cell differentiation through type I interferon and interleukin 6. Immunity 19, 225–234. 48. , Tsang, M. , Steinman, R. , and Munz, C. (2002) Human dendritic cells activate resting natural killer (NK) cells and are recognized via the NKp30 receptor by activated nk cells.
The cellular interaction kinetic rates, life spans, delays, activation thresholds) and analysis (the “numbers game”), and to make testable predictions in advance of experiments and clinical applications. From: Methods in Molecular Medicine, vol. 109: Adoptive Immunotherapy: Methods and Protocols Edited by: B. Ludewig and M. W. , Totowa, NJ 19 20 Bocharov, Ford, and Ludewig Table 1 Decision Making Before and During the Modeling Process a Modeling objectives Determine the focus of the investigation.
67 Nestle, F. , and Hart, D. (2001) Dendritic cells: on the move from bench to bedside. Nat. Med. 7, 761–765. 68. 68 Ashley, D. , Hale, L. , Bigner, D. , and Gilboa, E. (1997) Bone marrow-generated dendritic cells pulsed with tumor extracts or tumor RNA induce antitumor immunity against central nervous system tumors. J. Exp. Med. 186, 1177–1182. 69. 69 Celluzzi, C. M. and Falo, L. , Jr. (1998) Physical interaction between dendritic cells and tumor cells results in an immunogen that induces protective and therapeutic tumor rejection.
Adoptive immunotherapy : methods and protocols by Burkhard Ludewig, Matthias W. Hoffmann