By Curtis A. Williams, Merrill W. Chase
Equipment in Immunology and Immunochemistry, quantity IV: Agglutination, supplement, Neutralization, and Inhibition presents details pertinent to direct and oblique agglutination reactions. This publication covers numerous subject matters, together with complement-fixation approaches, isolation of supplement parts, hemolytic intermediates, complement-related proteins, and neutralization reactions.
Organized into 3 chapters, this quantity starts with an summary of test-tube agglutinations which are most popular for blood grouping with saline agglutinins that require quite a lot of mins for agglutination. this article then describes blood workforce antibodies that agglutinate crimson blood cells suspended in saline. different chapters think about the classical pathway of supplement usage. This e-book discusses besides the complexity of occasions resulting in hemolysis of erythrocytes through supplement. the ultimate bankruptcy offers with the power of antitoxin to neutralize diphtheria toxin and explains the quantitative relationships among antigen and antibody.
This ebook is a invaluable source for immunologists, scientists, and learn staff.
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Equipment in Immunology and Immunochemistry, quantity IV: Agglutination, supplement, Neutralization, and Inhibition offers info pertinent to direct and oblique agglutination reactions. This booklet covers various themes, together with complement-fixation techniques, isolation of supplement parts, hemolytic intermediates, complement-related proteins, and neutralization reactions.
Additional resources for Agglutination, Complement, Neutralization, and Inhibition
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Stavitsky, Immunology 12, 431 (1967). c] each tube. The same volume of sahne is added to each tube of a second control series. The same volume of various known concentrations of the standard antigen are added to tubes of additional series. All tubes are incubated overnight at room temperature. 05 ml volumes) is then added to all tubes and the control tubes, and the amount of protein in the unknown is estimated by comparison of its inhibition titer with that of the standard antigen. Alternatively, serial dilutions are made of the unknown and standard proteins in dilute, in activated, and absorbed normal rabbit serum.
Watson, / . Biol Chem. 140, 55 (1941). '^' G. Middlebrook, personal communication (1968). * E . Sorkin and S. V. Boyden, J. Immunol. 76, 22 (1955). ^ E . Sorkin, S. V. Boyden, and J . M . Rhodes, Helv. Chim. Acta 89, 1684 (1956). 8 F . B . Seibert, Amer. Rev. Tuherc. 69, 86 (1949). b] A large and diffuse body of literature has developed on the spectrum of the microbial antigens taken u p directly by erythrocytes. T h e abihty to adsorb to erythrocytes appears to involve patches of the polysaccharide surfaces other than those bearing the specific antibody-receptor.
Agglutination, Complement, Neutralization, and Inhibition by Curtis A. Williams, Merrill W. Chase