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C. Synthesis of the N-carboxyanhydride of Ȗ–benzyl lglutamate (BLG-NCA) Ȗ-benzyl l-glutamate was synthesized according to the reported method [8]. D. Synthesis of poly(ethylene glycol)-g-polyethylenimine-bpoly(Ȗ-benzyl l-glutamate) (PEG-g-PEI-b-PBLG) PEG-g-PEI-b-PBLG was prepared by ring opening polymerization of the BLG-NCA using synthesized PEG-gPEI as a macromolecule initiator in benzene/dioxane (v/v 1/4) according to a similar procedure [9] and [10]. E. Synthesis of PEG-g-PEI-b-poly(dimethylaminoethyl lglutamine) (PEG-g-PEI-b-PDMAEG) Synthesis of PEG-g-PEI-b-PDMAEG was conducted by aminolysis of PEG-g-PEI-b-PBLG using 2-Dimethylaminoethylamine in a flask equipped with a magnetic stir bar and a programmable temperature controller.

1. Materials pullulan (Mw 200 000) was purchased from Hayashibara Tokyo. (Japan). 4-dimethylaminopyridine (DMAP) and 1-ethyl-3-[3-(dimethylamino) propyl] carbodiimide (EDC) were purchased from Sigma Co. (St. Loius, MO, USA); pyrene were purchased from Aldrich Co. (Milwaukee, WI). All other chemical reagents were of analytical grade and obtained from commercial sources. ): APCMBE 2008, IFMBE Proceedings 19, pp. 2. 5. Preparation of self-assembled nanoparticals Synthesis was carried out according to the method reported by Shaikh VAE [29].

Carbohydrate Polymers, 66, 514-520. Lee, K. , Kwon, I. , & Jeong, S. Y. (2000). Selfaggregates of deoxycholic acid-modified chitosan as a novel carrier of adriamycin. Colloids Polymer Science, 278, 1216-1219. IFMBE Proceedings Vol. 19 _________________________________________________________________ Preparation and physicochemical characteristics of self-assembled nanoparticles of cholesterol succinate modified pullulan conjugates 18. Yuan, X. , & Yuan, Y. B. (2006). Preparation of cholesterolmodified chitosan self-aggregates for delivery of drugs to ocular surface.

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